Lipolytic Enzymes Produced by Aspergillus oryzae (II)
نویسندگان
چکیده
منابع مشابه
Induction of enzymes in dormant spores of Aspergillus oryzae.
In dormant conidia of Aspergillus oryzae, alpha-amylase, invertase, and glucose dehydrogenase were induced by their respective inducers. Neither germination nor swelling occurred during this period.
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Aspergillus oryzae, which is widely used for Japanese traditional fermentation, produced at least two lipolytic enzymes (L1 and L2). Southern hybridization analysis of restriction enzyme-digested genomic DNA fragments of Aspergillus oryzae with 23-mer oligonucleotides synthesized according to the amino acid sequence of the enzyme L1 as probes suggested that there is single copy of the L1 gene i...
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Solid-state fermentation (SSF) was carried out to produce α-amylase from Aspergillus oryzae (IFO 30103) using spent brewing grains (SBG) as substrate. A maximum of 11296 U/gds amylase activity was obtained after 48 h of fermentation. The extracted enzyme was subjected to partial purification by ammonium sulphate fractionation. Maximum specific activity was obtained with 40-70% fraction. SDS-PAG...
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ژورنال
عنوان ژورنال: JOURNAL OF THE BREWING SOCIETY OF JAPAN
سال: 1995
ISSN: 0914-7314,2186-4012
DOI: 10.6013/jbrewsocjapan1988.90.828